The Effect of Plant Growth Regulators on Shoot Regeneration of Two Wild Almond Species Amygdalus scoparia L. and Amygdalus lycioides Spach.

Document Type : Original Article

Authors

1 Higher Education Institute of Jahad University of Kermanshah, Kermanshah, Iran

2 Crop and Horticultural Science Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kermanshah, Iran

3 Department of Horticultural Sciences and Engineering, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran

Abstract

Two species of Amygdalus scoparia L. and A. lycioides Spach. from the Rosaceae family can be found in suitable environmental conditions due to their high resistance to drought and heat and resistance to calcareous soils and attacking nematodes and diseases. The propagation of fruit tree rootstocks through vegetative means can be enhanced through the optimization of micropropagation techniques. This research was done in plant tissue culture laboratory of the Agricultural and Natural Resources Research Center of Kermanshah in 2018. In this research, the improved micropropagation protocol of these plants, explants of disinfected nodes in MS medium in combination with BA (6-Benzyladenine) hormones, at 6 levels (0, 0.25, 0.5, 1, 2, and 3 mg L-1) in four levels of Indole Butyric Acid (0, 0.1, 0.25 and 0.5 mg L-1). The experiment was carried out with four replications in a completely randomized factorial design. The results indicated that the highest percentage of explant regeneration in the A. lyciodes genotype was achieved with a combination of 0.5 mg L-1 BA and 0.25 mg L-1 IBA, while in the A. scoparia genotype, the highest regeneration percentage was observed with a combination of 2 mg L-1 BA and 0 mg L-1 IBA. The A. lyciodes genotype exhibited the highest number of shoots when treated with 0.5 mg L-1 BA and 0.25 mg L-1 IBA, while the A. scoparia genotype showed the maximum shoot number at a BA concentration of 2 mg L-1 and IBA concentration of 0 mg L-1.

Graphical Abstract

The Effect of Plant Growth Regulators on Shoot Regeneration of Two Wild Almond Species Amygdalus scoparia L. and Amygdalus lycioides Spach.

Highlights

  • Iran is a key region globally for the origin and diversification of wild almond species.
  • Amygdalus lycioides and Amygdalus scoparia exhibit resistance to drought and calcareous soils.
  • A. scoparia and A. lycioides are crucial species utilized as almond rootstocks in arid conditions.
  • Due to challenges in rooting wild almonds, tissue culture propagation offers an efficient alternative for commercial propagation of rootstocks and cultivars.

Keywords

Main Subjects

References

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Agrotechniques in Industrial Crops
Volume 5, Issue 1
Agrotechniques in Industrial Crops (Agrotech Ind Crops) (Online ISSN: 2783-2945)
March 2025
Pages 50-56

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