Study of Essential Oil and Antioxidant Capacity of Clematis ispahanica Boiss.

Document Type : Original Article

Authors

1 Department of Rangeland Management, Faculty of Natural Resources, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Horticultural Science, Tarbiat Modares University, Tehran, Iran

3 Medicinal Plants Research Center, Institute of Medicinal Plants, Tehran, Iran

Abstract

Clematis ispahanica Boiss. is a rare species belonging to the Ranunculaceae family. Clematis ispahanica is highly valued for its ornamental and medicinal properties. The specific traditional uses of Clematis ispahanica in traditional medicine may include curing neurological disorders, syphilis, malaria, rheumatism, gout, diarrhea, and asthma. In this study, leaves of C. ispahanica were collected from collected from Boanat, Kerman Province, Iran. Essential oil isolated from the leaves of C. ispahanica was evaluated for its chemical composition for the first time. GC/MS analyzed the essential oil obtained by hydrodistillation. The chemical analysis of the oil from Clematis ispahanica Boiss. revealed the identification of 50 compounds, accounting for 92.5% of the total oil composition. In the leaves oil, the main components detected were phthalic acid, heptacosane, pentadecanoic acid, methyl ester, and a-pinene with amounts of 51.1 %, 5.8 %, 3.9 %, and 2.9 %, respectively. The analysis of various extracts from Clematis ispahanica Boiss. revealed a significant difference among all of them in Antioxidant activity (AA), Total phenol content (TPC) and Total flavonoid content (TFC) (p≤0.01, p≤0.05). For the antioxidant activity, the results obtained showed that the highest radical scavenging activity was observed in the extract of hexane with IC50 122 μg/ml, and the lowest activity was found in acetone extraction with IC50 170 μg/ml. The highest TPC value was 15.0 mg GAE/g DW in the extract of hexane. The highest TFC belongs to the extract of hexane and methanol with 7.3 mg QE/g and 7.2 mg QE/g. The analysis between TPC (r= 0.708, p≤0.01) and TFC (r= 0.786, p≤0.01) of C. ispahanica and its antioxidant properties were found to have a strong correlation. C. ispahanica exhibits a relatively high level of antioxidant potency and contains a significant amount of total phenolic compounds when extracted with a hexane solvent.

Graphical Abstract

Study of Essential Oil and Antioxidant Capacity of Clematis ispahanica Boiss.

Highlights

  • Essential oil and antioxidant activity of Clematis ispahanica Boiss were evaluated.
  • Total phenol content (TPC) and Total flavonoid content (TFC)  of Clematis ispahanica Boiss were assessed.
  • Positive correlation between the total phenolic content and antioxidant activity of all extracts of Clematis ispahanica Boiss was observed.

Keywords

Main Subjects

References

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Agrotechniques in Industrial Crops
Volume 4, Issue 4
Agrotechniques in Industrial Crops (Agrotech Ind Crops) (Online ISSN: 2783-2945)
December 2024
Pages 197-204

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