Qualitative Evaluation and Biological Effects of the Leaf and Bulb Heat-stable Proteins in Two Garlic Clones (Allium sativum)

Document Type : Original Article

Authors

1 Department of Plant Breeding, Bu-Ali Sina University, Hamedan, Iran

2 Department of Plant Protection, Bu-Ali Sina University, Hamedan, Iran

Abstract

Due to pathogen resistance as well as the high costs and adverse environmental effects of the use of chemical pesticides, researchers are looking for other ways to control pests and diseases such as biological control. Many studies prove the antibacterial effects of the biochemical compounds of the garlic plant, but there is no report on the antimicrobial activities of the heat-stable protein of the garlic plant. Considering the proven role of these proteins in response to stresses, this study was conducted to investigate the antibacterial role of these proteins on Ralstonia solanacearum and Streptomyces scabies bacteria. The antimicrobial properties of each bacterium were tested in separate experiments using a completely randomized factorial design with three factors and three repeats. Heat-stable protein from Clones, tissues, and various concentrations applied to R. solanacearum bacteria expressed a highly significant difference in the diameter of the inhibition zone and The highest inhibition zone was related to the leaf of Hamadan Clone. The lowest minimum inhibitory concentration MIC and minimal bactericidal concentration MBC were related to the leaf HSP of the Hamadan clone. As a result, Hamadan leaves with smaller MIC and MBC and the larger inhibitory zone in relation to the other treatments showed the highest inhibitory effect. In SDS-PAGE electrophoresis the leaves heat-stable protein electrophoresis banding only the HSP40 family was observed, while, on the garlic cloves families, small HSP (sHSP), HSP40, HSP70, HSP90, and HSP100 were seen. The results indicate that heat-stable protein from garlic could be used as a major antimicrobial agent against pathogenic R. solanacearum bacteria but had no biological role as an antimicrobial on S. scabies bacteria. The results of the present research show that the HSP of garlic plants can be used to create resistance to R. solanacearum bacteria.

Graphical Abstract

Qualitative Evaluation and Biological Effects of the Leaf and Bulb Heat-stable Proteins in Two Garlic Clones (Allium sativum)

Highlights

  • Heat-stable protein of the garlic plant has an effective role in inhibiting the growth of Ralstonia solanacearum bacteria.
  • The antimicrobial effect of heat-stable protein increases with increasing protein concentration.
  • The comparison of MIC and MBC results is similar to the results of the bacterial growth inhibition zone.
  • In leaves, only heat-stable protein electrophoresis bands of the HSP40 family were observed, whereas, in the garlic cloves families, small HSP (sHSP), HSP40, HSP70, HSP90 and HSP100 were seen.

Keywords

Main Subjects

References

Ahmad M., Tahir A., Anjum R.S. 2020. Heat stability of proteins and its exploitation for purification of heat-stable proteins. https://doi.org/10.20944/preprints202008.0225.v1
Al Akeel R., Al-Sheikh Y., Mateen A., Syed R., Janardhan K., Gupta V.C. 2014. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi Journal of Biological Sciences 21(2): 147-151. https://doi.org/10.1016/j.sjbs.2013.09.003
Asghari Jafarabadi M., Mohammadi S.M. 2015. Statistics series: common nonparametric methods. Iranian Journal of Diabetes and Metabolism 14(3): 145-162. (In Farsi). http://ijdld.tums.ac.ir/article-1-5364-fa.html
Bernstein H.D. 2019. Type V secretion in Gram-negative bacteria. EcoSal Plus 8(2). https://doi.org/10.1128/ecosalplus.esp-0031-2018
Bin C., Al-Dhabi N.A., Esmail G.A., Arokiyaraj S., Arasu M.V. 2020. Potential effect of Allium sativum bulb for the treatment of biofilm forming clinical pathogens recovered from periodontal and dental caries. Saudi Journal of Biological Sciences 27(6): 1428-1434. https://doi.org/10.1016/j.sjbs.2020.03.025
Bolhassani A., Agi E. 2019. Heat shock proteins in infection. Clinica Chimica Acta 498: 90-100. https://doi.org/10.1016/j.cca.2019.08.015
Brown L., Wolf J.M., Prados-Rosales R., Casadevall A. 2015. Through the wall: Extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews Microbiology 13(10): 620-630. https://doi.org/10.1038/nrmicro3480
Chung C.R., Kuo T.R., Wu L.C., Lee T.Y., Horng J.T. 2020. Characterization and identification of antimicrobial peptides with different functional activities. Briefings in Bioinformatics 21(3): 1098-1114. https://doi.org/10.1093/bib/bbz043
Dini I., De Biasi M.G., Mancusi A. 2022. An overview of the potentialities of antimicrobial peptides derived from natural sources. Antibiotics 11(11): 1483. https://doi.org/10.3390/antibiotics11111483
Dong J., Ding X., Wang S. 2019. Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography. BMC Biotechnology 19(1): 1-8. https://doi.org/10.1186/s12896-019-0590-y
Drira M., Ghanmi S., Zaidi I., Brini F., Miled N., Hanin M. 2023. The heat‐stable protein fraction from Opuntia ficus‐indica seeds exhibits an enzyme protective effect against thermal denaturation and antibacterial activity. Biotechnology and Applied Biochemistry 70(2): 593-602. https://doi.org/10.1002/bab.2382
Farha S., Chatterjee E., Manuel S.G., Reddy S.A., Kale R.D. 2012. Isolation and characterization of bioactive compounds from fruit wastes. Dynamic Biochemistry, Process Biotechnology and Molecular Biology 6: 92-94.
Gajic I., Kabic J., Kekic D., Jovicevic M., Milenkovic M., Mitic Culafic D., Trudic A., Ranin L., Opavski N. 2022. Antimicrobial susceptibility testing: A comprehensive review of currently used methods. Antibiotics 11(4): 427. https://doi.org/10.3390/antibiotics11040427
Gan B.H., Gaynord J., Rowe S.M., Deingruber T., Spring D.R. 2021. The multifaceted nature of antimicrobial peptides: Current synthetic chemistry approaches and future directions. Chemical Society Reviews 50(13): 7820-7880. https://doi.org/10.1039/D0CS00729C
Kaur S., Samota M.K., Choudhary M., Choudhary M., Pandey A.K., Sharma A., Thakur J. 2022. How do plants defend themselves against pathogens-Biochemical mechanisms and genetic interventions. Physiology and Molecular Biology of Plants 28(2): 485-504. https://doi.org/10.1007/s12298-022-01146-y
Kowalska-Krochmal B., Dudek-Wicher R. 2021. The minimum inhibitory concentration of antibiotics: Methods, interpretation, clinical relevance. Pathogens 10(2): 165. https://doi.org/10.3390/pathogens10020165
Liu J., Nothias L.F., Dorrestein P.C., Tahlan K., Bignell D.R. 2021. Genomic and metabolomic analysis of the potato common scab pathogen Streptomyces Scabiei. ACS Omega 6(17): 11474-11487. https://doi.org/10.1021/acsomega.1c00526
Manjula A.C., Shubha. 2011. Screening of antibacterial activity of total soluble protein of mulberry varieties. International Journal of Current Pharmaceutical Research 3(2): 60-61.
Matsumoto H., Haniu H., Komori N. 2019. Determination of protein molecular weights on SDS-PAGE. Electrophoretic Separation of Proteins: Methods and Protocols 1855: 101-105. https://doi.org/10.1007/978-1-4939-8793-1_10
Mirzaei Najafgholi H., Narimani S., Aeini M., Taghavi S.M., Tarighi S., Javaheri M. 2015. Investigation the performance and biological control of the various tomato cultivars against the bacterial wilt disease (Ralstonia solanacearum). Biocontrol in Plant Protection 2(2): 47-57. (In Farsi). https://doi.org/10.22092/bcpp.2015.103000
Mortazavi S.H., Azadmard Damirchi S., Sowti M., Mahmudi R., Safaeean F., Moradi Azad S. 2014. Antimicrobial Effects of ethanolic extract of the hull and the core of Pistacia khinjuk stocks. Innovative Food Technologies 1(4): 81-88. (In Farsi). https://doi.org/10.22104/jift.2014.46
Moussa Z., Rashad E.M., Elsherbiny E.A., Al-Askar A.A., Arishi A.A., Al-Otibi F.O., Saber W.I. 2022. New strategy for inducing resistance against bacterial wilt disease using an avirulent strain of Ralstonia solanacearum. Microorganisms 10(9): 1814. https://doi.org/10.3390/microorganisms10091814
Nazarov P.A., Baleev D.N., Ivanova M.I., Sokolova L.M., Karakozova M.V. 2020. Infectious plant diseases: Etiology, current status, problems and prospects in plant protection. Acta Naturae 12(3): 46. https://doi.org/10.32607%2Factanaturae.11026
Nourbakhsh F., Rezaei S. 2012. Heat shock proteins (HSPs). Andishe-Sara Press. 120 p. (in Farsi)
Park C.J., Seo Y.S. 2015. Heat shock proteins: a review of the molecular chaperones for plant immunity. The Plant Pathology Journal 31(4): 323-333. https://doi.org/10.5423/ppj.rw.08.2015.0150
Park H., Yamanaka T., Nukina N. 2022. Proteomic analysis of heat-stable proteins revealed an increased proportion of proteins with compositionally biased regions. Scientific Reports 12(1): 4347. https://doi.org/10.1038/s41598-022-08044-z
Pasquina-Lemonche L., Burns J., Turner R.D., Kumar S., Tank R., Mullin N., Wilson J.S., Chakrabarti B., Bullough P.A., Foster S.J., Hobbs J.K. 2020. The architecture of the Gram-positive bacterial cell wall. Nature 582(7811): 294-297. https://doi.org/10.1038/s41586-020-2236-6
Saif S., Hanif M.A., Rehman R., Riaz M. 2020. Garlic. Medicinal Plants of South Asia (pp. 301-315). Elsevier. https://doi.org/10.1016/B978-0-08-102659-5.00023-9
Sarkar T., Chetia M., Chatterjee S. 2021. Antimicrobial peptides and proteins: From nature’s reservoir to the laboratory and beyond. Frontiers in Chemistry 9: 691532. https://doi.org/10.3389/fchem.2021.691532
Singhai P.K., Sarma B.K., Srivastava J.S. 2011. Biological management of common scab of potato through Pseudomonas species and vermicompost. Biological Control 57(2): 150-157. https://doi.org/10.1016/j.biocontrol.2011.02.008
ul Haq S., Khan A., Ali M., Khattak A.M., Gai W.X., Zhang H.X., Wei A.M., Gong Z.H. 2019. Heat shock proteins: dynamic biomolecules to counter plant biotic and abiotic stresses. International journal of molecular sciences 20(21): 5321. https://doi.org/10.3390/ijms20215321
Wang W., Vinocur B., Shoseyov O., Altman A. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 9(5): 244-252. https://doi.org/10.1016/j.tplants.2004.03.006
Agrotechniques in Industrial Crops
Volume 4, Issue 1
Agrotechniques in Industrial Crops (Agrotech Ind Crops) (Online ISSN: 2783-2945)
March 2024
Pages 16-23

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