Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant

Document Type : Original Article

Authors

1 Department of Genetic and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is 40=2n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, 224 WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, 2 genes WRKY8 and WRKY57 under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina.

Graphical Abstract

Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant

Highlights

  • WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses.
  • In this research, the WRKY gene family was investigated in two lines sensitive and tolerant to drought stress under drought stress conditions.
  • Two genes were highly expressed in drought-tolerant varieties under drought conditions, while no obvious expression failure was observed in sensitive varieties.
  • The results of this research have provided suitable information for the evolutionary study and function of the WRKY gene family in Camelina and can be a start for further studies on the role of the WRKY gene family in Camelina.

Keywords

Main Subjects

References

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Agrotechniques in Industrial Crops
Volume 4, Issue 2
Agrotechniques in Industrial Crops (Agrotech Ind Crops) (Online ISSN: 2783-2945)
June 2024
Pages 65-79

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