Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant

Document Type : Original Article

Authors

1 Department of Genetic and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is 40=2n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, 224 WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, 2 genes WRKY8 and WRKY57 under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina.

Graphical Abstract

Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant

Highlights

  • WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses.
  • In this research, the WRKY gene family was investigated in two lines sensitive and tolerant to drought stress under drought stress conditions.
  • Two genes were highly expressed in drought-tolerant varieties under drought conditions, while no obvious expression failure was observed in sensitive varieties.
  • The results of this research have provided suitable information for the evolutionary study and function of the WRKY gene family in Camelina and can be a start for further studies on the role of the WRKY gene family in Camelina.

Keywords

Main Subjects

References

Abdullah H.M., Akbari P., Paulose B., Schnell D., Qi W., Park Y., Pareek A., Dhankher O.P. 2016. Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds. Biotechnology for Biofuels 9: 136.‏ https://doi.org/10.1186/s13068-016-0555-5
Brock J.R., Donmez A.A., Beilstein M.A., Olsen K.M. 2018. Phylogenetics of Camelina crantz. (Brassicaceae) and insights on the origin of gold-of-pleasure (Camelina sativa). Molecular Phylogenetics and Evolution 127: 834-842.‏ https://doi.org/10.1016/j.ympev.2018.06.031
Chen J., Nolan T.M., Ye H., Zhang M., Tong H., Xin P., Chu J., Chu C., Li Z., Yin Y. 2017. Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved in brassinosteroid-regulated plant growth and drought responses. The Plant Cell 29(6): 1425-1439. https://doi.org/10.1105/tpc.17.00364
Christou A., Georgiadou E.C., Filippou P., Manganaris G.A., Fotopoulos V. 2014. Establishment of a rapid, inexpensive protocol for extraction of high quality RNA from small amounts of strawberry plant tissues and other recalcitrant fruit crops. Gene 537(1): 169-173. https://doi.org/10.1016/j.gene.2013.11.066
Guo H., Wang Y., Wang L., Hu P., Wang Y., Jia Y., Zhang C., Zhang Y., Zhang Y., Wang C., Yang C. 2017. Expression of the MYB transcription factor gene Bpl MYB 46 affects abiotic stress tolerance and secondary cell wall deposition in Betula platyphylla. Plant Biotechnology Journal 15(1): 107-121.‏ https://doi.org/10.1111/pbi.12595
He G.H., Xu J.Y., Wang Y.X., Liu J.M., Li P.S., Chen M., Ma Y.Z., Xu Z.S. 2016a. Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis. BMC Plant Biology 16: 1-6. https://doi.org/10.1186/s12870-016-0806-4
He Y., Mao S., Gao Y., Zhu L., Wu D., Cui Y., Li J., Qian W. 2016b. Genome-wide identification and expression analysis of WRKY transcription factors under multiple stresses in Brassica napus. PloS One 11(6): e0157558. https://doi.org/10.1371/journal.pone.0157558
Heydarian Z., Yu M., Gruber M., Coutu C., Robinson S.J., Hegedus D.D. 2018. Changes in gene expression in Camelina sativa roots and vegetative tissues in response to salinity stress. Scientific Reports 8(1): 9804. https://doi.org/10.1038/s41598-018-28204-4
Hussain R.M., Kim H.K., Khurshid M., Akhtar M.T., Linthorst H.J. 2018. Overexpression of AtWRKY50 is correlated with enhanced production of sinapic derivatives in Arabidopsis. Metabolomics 14: 1-11.‏ https://doi.org/10.1007/s11306-018-1317-0
Ji C., Mao X., Hao J., Wang X., Xue J., Cui H., Li R. 2018. Analysis of bZIP transcription factor family and their expressions under salt stress in Chlamydomonas reinhardtii. International Journal of Molecular Sciences 19(9): 2800. https://doi.org/10.3390/ijms19092800
Jiang Y., Guo L., Ma X., Zhao X., Jiao B., Li C., Luo K. 2017. The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus. Tree Physiology 37(5): 665-675.‏ https://doi.org/10.1093/treephys/tpx008
Jing Z., Liu Z. 2018. Genome-wide identification of WRKY transcription factors in kiwifruit (Actinidia spp.) and analysis of WRKY expression in responses to biotic and abiotic stresses. Genes & Genomics 40: 429-446.‏ https://doi.org/10.1007/s13258-017-0645-1
Li C., Li D., Shao F., Lu S. 2015. Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza. BMC Genomics 16(1): 1-21.‏ https://doi.org/10.1186/s12864-015-1411-x
Li D., Liu P., Yu J., Wang L., Dossa K., Zhang Y., Zhou R., Wei X., Zhang X. 2017. Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses. BMC Plant Biology 17(1): 1-19.‏ https://doi.org/10.1186/s12870-017-1099-y
Moser B.R. 2010. Camelina (Camelina sativa L.) oil as a biofuels feedstock: Golden opportunity or false hope?. Lipid Technology 22(12): 270-273. https://doi.org/10.1002/lite.201000068
Neupane D., Lohaus R.H., Solomon J.K.Q., Cushman J.C. 2022. Realizing the potential of Camelina sativa as a bioenergy crop for a changing global climate. Plants (Basel) 11(6): 772. https://doi.org/10.3390%2Fplants11060772
Ng D.W., Abeysinghe J.K., Kamali M. 2018. Regulating the regulators: the control of transcription factors in plant defense signaling. International Journal of Molecular Sciences 19(12): 3737. https://doi.org/10.3390%2Fijms19123737
Raineri J., Wang S., Peleg Z., Blumwald E., Chan R.L. 2015. The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress. Plant Molecular Biology 88: 401-413.‏ https://doi.org/10.1007/s11103-015-0329-7
Rasouli H., Fazeli-Nasab B. 2014. Structural validation and homology modeling of LEA 2 protein in bread wheat. American-Eurasian Journal of Agricultural & Environmental Sciences 14(10): 1044-1048. http://dx.doi.org/10.5829/idosi.aejaes.2014.14.10.12424 
Rinerson C.I., Rabara R.C., Tripathi P., Shen Q.J., Rushton P.J. 2015. The evolution of WRKY transcription factors. BMC Plant Biology 15: 1-18.‏ https://doi.org/10.1186/s12870-015-0456-y
Rushton P.J., Somssich I.E., Ringler P., Shen Q.J. 2010. WRKY transcription factors. Trends in Plant Science 15(5): 247-258.‏ https://doi.org/10.1016/j.tplants.2010.02.006
Shi W.Y., Du Y.T., Ma J., Min D.H., Jin L.G., Chen J., Chen M., Zhou Y.B., Ma Y.Z., Xu Z.S., Zhang X.H. 2018. The WRKY transcription factor GmWRKY12 confers drought and salt tolerance in soybean. International Journal of Molecular Sciences 19(12): 4087. https://doi.org/10.3390/ijms19124087
Song H., Wang P., Hou L., Zhao S., Zhao C., Xia H., Li P., Zhang Y., Bian X., Wang X. 2016a. Global analysis of WRKY genes and their response to dehydration and salt stress in soybean. Frontiers in Plant Science 7: 9.‏ https://doi.org/10.3389%2Ffpls.2016.00009
Song H., Wang P., Lin J.Y., Zhao C., Bi Y., Wang X. 2016b. Genome-wide identification and characterization of WRKY gene family in peanut. Frontiers in Plant Science 7: 534.‏ https://doi.org/10.3389%2Ffpls.2016.00534
Song M., Peng X. 2019. Genome-wide identification and characterization of DIR genes in Medicago truncatula. Biochemical Genetics 57: 487-506.‏ https://doi.org/10.1007/s10528-019-09903-7
Tang J., Wang F., Hou X.L., Wang Z., Huang Z.N. 2014. Genome-wide fractionation and identification of WRKY transcription factors in Chinese cabbage (Brassica rapa ssp. pekinensis) reveals collinearity and their expression patterns under abiotic and biotic stresses. Plant Molecular Biology Reporter 32: 781-795.‏ https://doi.org/10.1007/s11105-013-0672-2
Vafaei N., Tavakolipour H., Ghodsvali A.R. 2010. Some biophysical properties of oily sunflower achenes in Golestan province. Journal of food science and technology 7(25): 103-115. (In Farsi). http://fsct.modares.ac.ir/article-7-6163-en.html
Wang M., Tu L., Yuan D., Zhu D., Shen C., Li J., Liu F., Pei L., Wang P., Zhao G., Ye Z. 2019. Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense. Nature Genetics 51(2): 224-229. https://doi.org/10.1038/s41588-018-0282-x
Wei Y., Shi H., Xia Z., Tie W., Ding Z., Yan Y., Wang W., Hu W., Li K. 2016. Genome-wide identification and expression analysis of the WRKY gene family in cassava. Frontiers in Plant Science 7: 25.‏ https://doi.org/10.3389/fpls.2016.00025
Wu J., Chen J., Wang L., Wang S. 2017. Genome-wide investigation of WRKY transcription factors involved in terminal drought stress response in common bean. Frontiers in Plant Science 8: 380.‏ https://doi.org/10.3389/fpls.2017.00380
Xie T., Chen C., Li C., Liu J., Liu C., He Y. 2018. Genome-wide investigation of WRKY gene family in pineapple: evolution and expression profiles during development and stress. BMC Genomics 19: 1-18.‏ https://doi.org/10.1186/s12864-018-4880-x
Yang X., He K., Chi X., Chai G., Wang Y., Jia C., Zhang H., Zhou G., Hu R. 2018. Miscanthus NAC transcription factor MlNAC12 positively mediates abiotic stress tolerance in transgenic Arabidopsis. Plant Science 277: 229-241. https://doi.org/10.1016/j.plantsci.2018.09.013
Yuan L., Mao X., Zhao K., Ji X., Ji C., Xue J., Li R. 2017. Characterization of phospholipid: diacylglycerol acyltransferases (PDATs) from Camelina sativa and their roles in stress responses. Biology Open 6(7): 1024-1034.‏ https://doi.org/10.1242/bio.026534
Zheng J., Liu F., Zhu C., Li X., Dai X., Yang B., Zou X., Ma Y. 2019. Identification, expression, alternative splicing and functional analysis of pepper WRKY gene family in response to biotic and abiotic stresses. PloS One 14(7): e0219775.‏ https://doi.org/10.1371/journal.pone.0219775
Agrotechniques in Industrial Crops

Articles in Press, Accepted Manuscript
Available Online from 01 February 2024

Files

Share

How to cite

Statistics