Cluster Analysis Helps to Select Appropriate Pollination States by Crossing of Phalaenopsis Varieties

Document Type : Original Article


Department of Agriculture and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran


To study the effective morphological and physiological characteristics and the cognition of main factors affecting pollination yield and seed production of phalaenopsis orchid, five varieties and their crossing were studied in a greenhouse of the Agriculture Institute, Research Institute of Zabol on 2019-2022. Eight morphological and physiological characteristics of pollination were evaluated in this research for three years and their grouping was according to a completely randomized design. The results of cluster analysis with the method of UPGMA based on the maximum distance between the clusters, 25 pollination states were divided into two groups. The results of detection of function analysis significantly correlated with cluster analysis. Also, factors analysis showed that two main factors fitted 77.1% of the data variation considered in the breeding of characteristics with the most variations in each factor. Morphological characteristics such as fresh weight of capsule (FWC), capsule volume (CV), and weight of seed in capsule (WSC) had the most positive variations between factors, and physiological traits such as TCS (Time to Capsule formation Sign) and TPS (Time to first Pollination Sign) showed the most negative one. The most amounts of FWC, CV, and WSC characteristics belonged to Nottingham× Nottingham with 9.66 g, 23.5 cm3, and 3.58 g, respectively and in the cross-pollination of Andorra× Nottingham with 8.67 g, 20.8 cm3, and 3.4 g respectively. The results of the factors analysis showed the importance of morphological pollination characteristics (FWC, CV, and WSC) and physiological traits (TPS and TCS) in the indirect selection of desired genotypes for pollination of orchid varieties. 

Graphical Abstract

Cluster Analysis Helps to Select Appropriate Pollination States by Crossing of Phalaenopsis Varieties


  • Varieties and hybrids were divided into two main groups by cluster analysis.
  • Cross-pollination altered morphological and physiological characteristics, separating the average characteristics of self-pollination states from cross-pollination.
  • The desired characteristics were CV, WSC, and TPS based on the factor analysis.
  • The selection of parents' quality is a very important factor in determining the success of the hybridization program.


Main Subjects

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