Evaluation of Dormancy Failure Datura stramonium Plant Seeds under the Influence of Different Treatments

Document Type : Original Article


1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

2 Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

3 Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran


The seeds of most medicinal plants have a variety of dormancy. Therefore, it is necessary to know the effective methods of breaking the seed dormancy for their production and cultivation. In this study, to determine the best method of breaking dormancy and increasing the germination percentage of tattoo seeds, various factors have been investigated. Different treatments, scratching with sandpaper, embryo culture, GA treatment (100, 150 and 200 PPM) and sulfuric acid treatment (50, 75 and 100%) with filter paper or on MS medium containing BAP (0.5, 1 and 1.5 mg/l) was applied with untreated samples (control). Embryo culture was performed only on MS medium containing BAP hormone. The analysis of variance showed that all interactions with the studied treatments were significantly different in terms of germination percentage, fresh and dry weight of roots and stems (P <0.01). It was observed that the use of benzyl aminopurine (1.5 mg/l) with gibberellic acid (200 ppm) increased the seed germination percentage compared to the control. The use of benzylaminopurine (1.5 mg/l) and sulfuric acid (50%) significantly increased seed germination compared to the control. It was concluded that the effect of GA with BAP on seed germination was greater than sulfuric acid with BAP.

Graphical Abstract

Evaluation of Dormancy Failure Datura stramonium Plant Seeds under the Influence of Different Treatments


  • The scratching is a necessary factor to eliminate the dormancy of Datura seeds.
  • The simultaneous use of scratching + GA, sulfuric acid, and Benzylaminopurine improved the germination of the seeds.
  • The use of MS medium as a substrate for seed dormancy treatment was superior to filter paper.



Main Subjects

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